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Ultrafast Laser Offers Fully Flexible Dispersion Control for MPE Imaging

Fri, 02/25/2011 - 5:02am

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Ultrafast Laser Offers Fully Flexible Dispersion Control for MPE ImagingThe Chameleon Vision-S from Coherent, Inc. sets a new standard for multiphoton imaging by combining short (80 fs) pulsewidths with the industry’s highest average power and longest wavelength tuning range. Specifically, it delivers more than 410 kW of usable peak power—after GVD precompensation—and hands-free tuning from 690 to 1050 nm. The blend of short pulsewidths and high average power translate into the highest precompensated peak power currently available from any “one-box” laser.

The Chameleon Vision-S is an ideal laser for multiphoton excitation (MPE) imaging for several reasons. The laser’s high peak power delivers maximum imaging brightness in deeper tissues. Moreover, because the Chameleon Vision-S is a true one-box laser with the laser oscillator and GVD precompensator integrated on a single baseplate, it also provides unmatched beam pointing stability (80 microrad per 100 nm of tuning) which is another prerequisite for deep tissue imaging.

The laser’s Group Velocity Dispersion (GVD) precompensator provides seamless, hands-free adjustment of negative dispersion from zero up to a maximum of 43,000 fs2—enabling unmatched pulse duration flexibility at the sample, even in next generation microscopes with multiple AO modulators. These next generation microscopes provide fast scanning of selected image sectors in applications such as neuroscience that require high frame rates to follow dynamic events in real-time. Plus the circular TEM00 Gaussian output beam (M2 < 1.1) maximizes image resolution at any tissue depth.

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